About the Project
The aetiology of FSGS is still unknown although a major recent advance has been the identification of the podocyte as the target cell in this disease. Podocytes are part of the glomerular capillary wall of the kidney which is responsible for plasma ultrafiltration during primary urine formation and dysfunction of this process results in extensive leakage of plasma proteins – the nephrotic syndrome. Podocytes are terminally differentiated epithelial cells critical in preventing protein passage across the filtration barrier. This is achieved via a highly regulated and dynamic cytoskeleton, leading to maintenance of unique foot processes. Defects in podocytes play a major role in the pathogenesis of FSGS. Notably mutations in a number of cytoskeleton-regulating genes cause FSGS (5). We have developed a number of conditionally–immortalised human podocyte cell lines from both normal and diseased glomeruli from patients with known nephrotic syndrome disease causing mutations (6). We have also developed a novel 3D co-culture model of the glomerulus which provides a powerful model with which to understand the biology, and test new treatments using a patient specific precision medicine approach. We wish to use these unique resources and state of the art proteomic, cell biology and state of the art cell imaging techniques (both light and electron microscopy) to understand how mutations in specific podocyte proteins lead to disease.
Furthermore, we have the ability to test new and re-purposed drug compounds in these models to discover new treatment options.
2.Daskalakis, N. & Winn, M.P. Focal and segmental glomerulosclerosis: varying biologic mechanisms underlie a final histopathologic end point. Semin. Nephrol. (2006) 26, 89-94.
3.Franceschini, N. et al. NPHS2 gene, Nephrotic syndrome and focal segmental glomerulosclerosis: a HuGE review. Genet. Med. (2006) 8, 63-75.
4.Dragovic, D., Rosenstock, J.L., Wahl, S.J., Panagopoulos, G., DeVita, M.V. and Michelis, M.F. Increasing incidence of focal segmental glomerulosclerosis and an examination of demographic patterns. Clin. Nephrol. (2005) 63, 1-7
5. Chen, Y.M. & Liapism H. Focal segmental glomerulosclerosis: molecular genetics and targeted therapies. BMC Nephrol. (2015) 16:101.
6. Saleem, M.A., et al., A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol, 2002. 13(3): p. 630-8.
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