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Illuminating cell division: developing live cell imaging techniques to quantify cell cycle dynamics in plants

   Cardiff School of Biosciences

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  Dr AR Jones, Prof P Borri  No more applications being accepted  Funded PhD Project (UK Students Only)

About the Project

Cell division is fundamental to multicellular growth and development. Plants develop continuously and make many fine adjustments to cell division patterns to create organs that are perfectly adapted to a range of different functions and environment conditions. Understanding the rules of division – and how they can be broken – is an essential goal in fundamental biology. We are interested in learning how to make predictable changes to organ structure by making precise changes to cell division.

Plants are a beautiful model for cell division because cells are fixed in position and can be tracked over time for days. This has led to multiple fundamental advances in understanding cell size control, division plane orientation, and integration cell division with organ size and shape. However, to understand these processes better we need to be able to quantify processes more accurately. For example, we need to be able to quantify proteins that are expressed at low concentrations and rapidly turned over during the cell cycle. We would also like to be able to use label free label free techniques that look at intact samples without staining.

The aims of this project are to i) develop and validate an extended set of fluorescent cell cycle markers ii) develop methods to quantify small amounts of proteins using new fluorescent proteins iii) develop label-free methods for tracking the cell cycle.

We are an interdisciplinary group using a combination of cutting-edge live cell imaging, mathematical modelling and genetics to study plant development. If you are fascinated by beautiful patterns, plants and microscopes we would love to hear from you!

Please contact [Email Address Removed] for further information.

Funding Notes

Funding only covers Home UK student fees and stipend
EU and International Students may apply but will need to cover the difference between Home UK and International fees;

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