In vitro models of type 1 diabetes.
Type 1 diabetes is a condition in which the immune system destroys the cells that produce insulin, the hormone controlling the level of glucose in the blood stream. We have developed methods for generating immune cells and insulin producing cells from human pluripotent stem cells, providing an opportunity to directly observe interactions between beta cells and cells of the immune system in a person specific context. In this project, we will use stem cell models to investigate mechanisms underlying type 1 diabetes, with particular emphasis on the interaction between cells of the immune system and insulin producing beta cells.
This project will involve generating antigen presenting cells from induced pluripotent stem cells and testing their capacity to activate T-cells derived from individuals from type 1 diabetes. Students will learn how to grow, genetically modify and differentiate pluripotent stem cells. The project will also involve techniques such as flow cytometry, immunofluorescence, confocal microscopy, and quantitative PCR. Depending on the direction of the work, generation and analysis of RNAseq data may also be necessary.
The Immune Development Laboratory is physically located at the Murdoch Children’s Research Institute, part of the Melbourne Children’s Campus, which also includes the Royal Children’s Hospital and University of Melbourne: See our website for further information.
*To receive a reply to your inquiry, you should:
* Construct a short 4 sentence paragraph outlining why they interested in B-cell development and provide a single potential question that they would like to answer regarding the development of this cell type.
*Provide a copy of your CV
*Provide a copy of your academic transcripts
Students are enrolled through the Department of Paediatrics, University of Melbourne. Scholarships and Fee Waivers are available from the University on a competitive basis (https://scholarships.unimelb.edu.au). Students usually require a GPA of 85/100 in order to be competitive.English language requirements apply. Specifically, candidates need to have IELTS score of 7 overall with no individual task score being lower than 7.
* To receive a reply to your inquiry, applicants should:
* Provide a short 3 sentence paragraph outlining why you are interested in B-cell development.
* Provide a copy of their CV
* Provide a copy of their academic transcripts.
Publications from our laboratory can be seen via the publicly accessible Google Scholar profile of the supervisor (Ed Stanley):