The goals of this PhD student project are: 1) Investigate the histopathological and ultrastructural changes to astrocytes in mouse models and autopsy tissue of TBI. 2) Investigate the role of astrocytes in the driving and spreading of proteinopathies in the context of TBI 3) Evaluate single cell and secretomic profiles of astrocytes in mouse models and autopsy tissue across different stages of TBI sequelae
This project will involve the use of animal models of repetitive mild TBI, molecular biology and histopathological techniques, in vivo manipulations and ex vivo primary cell cultures. Molecular biology techniques include: immunoblotting, ELISA, flow cytometry, genomics, and proteomics. Histopathological techniques will involve: immunohistochemistry, immunofluorescence/confocal microscopy, electron microscopy and stereology approaches. In vivo and ex vivo studies will involve: administration of experimental TBI to mice, intranasal and intracerebral injections, brain inoculation, CSF collection and brain tissue dissection, culturing of primary adult astrocytes.
The Roskamp Institute, Inc., is a vibrant biomedical research organization in Sarasota, FL, that is focused on understanding the causes of, and developing treatments for, neurodegenerative and neuropsychiatric conditions including Alzheimer’s Disease, Traumatic Brain Injury and Posttraumatic Stress Disorder. The multidisciplinary and multicultural Institute includes basic, translational and applied research.