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  Investigating Platelet and Megakaryocyte Signalling Using Advanced Microscopy Techniques


   School of Pharmacy

  ,  Applications accepted all year round  Self-Funded PhD Students Only

About the Project

Investigating Platelet and Megakaryocyte Signalling Using Advanced Microscopy Techniques

Join the platelet biology research group in an exciting PhD project where you will benefit from specialist training and access to advanced microscopy facilities to investigate intracellular signalling processes in megakaryocytes and platelets. Megakaryocytes undergo thrombopoiesis (platelet production) to release millions of platelets into the circulation every day. Platelets form clots that are vital to prevent blood loss (haemostasis) but can also block arteries (thrombosis) and cause heart attacks and strokes. Some diseases such as obesity and diabetes increase the risk of thrombosis, and this may involve changes in the way megakaryocytes develop and produce platelets.

We have developed tools and methods to study platelet and megakaryocyte signalling and function and we now seek a motivated and enthusiastic student to undertake this important project.

The PhD candidates will have opportunities to learn a broad spectrum of modern state of the art techniques including but not limited to:

• Live-cell confocal microscopy

• Super-resolution microscopy

• Intravital (in vivo) microscopy

• Image analysis

• Gel electrophoresis and Western blotting

• Tissue culture of primary cells and cell lines

• Statistical analysis

• Platelet functional assays

• Flow cytometry

The Institute of Metabolic and Cardiovascular Research at the University of Reading bring together several established platelet research groups from across the School and Pharmacy and School of Biological Sciences to provide a thriving research environment. The student will have access to excellent facilities for the study of platelet and megakaryocyte biology as well as the supervision and mentorship of a community of scientists with shared interests.

Self-funded applicants should feel to get in touch, your enquiries will be welcome.


Biological Sciences (4)

Funding Notes

"Funding Notes
• Applicants should hold or expect to gain a first class (or minimum of a 2:1) bachelor’s degree in relevant disciplines in life sciences.
• Applicants must demonstrate excellent knowledge and some practical skills in cell biology.
• They should demonstrate good communication skills.
• Students with their own financial support are welcome to contact us at any time"

References

"Bye, A.P., et al., Pirtobrutinib results in reversible platelet dysfunction compared to ibrutinib and acalabrutinib. Haematologica, 2022.
Kriek, N., et al., Cucurbitacins Elicit Anti-Platelet Activity via Perturbation of the Cytoskeleton and Integrin Function. Thromb Haemost, 2022. 122(7): p. 1115-1129.
Alatawi, K.A., et al., 1,8-Cineole Affects Agonists-Induced Platelet Activation, Thrombus Formation and Haemostasis. Cells, 2021. 10(10).
Dunster, J.L., et al., Multiparameter phenotyping of platelet reactivity for stratification of human cohorts. Blood Adv, 2021. 5(20): p. 4017-4030.
Bye, A.P., et al., Aberrant glycosylation of anti-SARS-CoV-2 spike IgG is a prothrombotic stimulus for platelets. Blood, 2021. 138(16): p. 1481-1489.
Gaspar, R.S., et al., Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring. Sci Rep, 2021. 11(1): p. 1473.
Sahli, K.A., et al., Structural, functional, and mechanistic insights uncover the fundamental role of orphan connexin-62 in platelets. Blood, 2021. 137(6): p. 830-843.
Unsworth, A.J., et al., Antiplatelet properties of Pim kinase inhibition are mediated through disruption of thromboxane A2 receptor signaling. Haematologica, 2021. 106(7): p. 1968-1978.
Bye, A.P., J.M. Gibbins, and M.P. Mahaut-Smith, Ca(2+) waves coordinate purinergic receptor-evoked integrin activation and polarization. Sci Signal, 2020. 13(615)."

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