Additional supervisor Dr Amanda Tatler, University of Nottingham
Informal enquiries should be directed to the lead project supervisor [Email Address Removed]
Applicants should have a strong background in biological sciences. They should have a commitment to research in lung disease / immunology and hold or realistically expect to obtain at least an Upper Second Class Honours Degree in a relevant subject. Other key criteria include:
- A willingness to learn and apply new scientific techniques.
- An ability to analyse information and communicate effectively.
- An ability to work well within a team and independently when required.
- Previous experience with cell culture or flow cytometry is desirable, but not essential.
Progressive Pulmonary Fibrosis (PPF) is a devastating lung condition, which causes worsening breathlessness, disability, and early mortality. Early studies have implicated macrophage metabolic reprogramming and dysfunction in PPF pathogenesis. Extracellular Vesicles (EVs) have also been shown to transport pro-fibrotic cargo (including microRNAs) in models of PPF.
The aim of this studentship will be to determine the role of extracellular vesicles in mediating macrophage dysfunction and metabolic reprogramming in PPF. The objectives will be: 1) to characterise the functional and metabolic profile of macrophages from PPF patients; 2) to determine the biological effect of extracellular vesicles on PPF patient macrophages and on human lung slice models of fibrosis; 3) to determine whether these effects can be reversed.
This is an exciting and topical project, which could identify new therapeutic targets for PPF. A range of translational research skills will be taught, including flow cytometry, fluorescence microscopy, qPCR, Seahorse metabolic profiling, macrophage functional assays, microRNA profiling, extracellular vesicle characterisation and human lung slice models. This project benefits from access to PPF patient samples and human lung tissue from surgical resections. You will work within an integrated collaborative research team of discovery scientists and clinical academics in the Institute of Inflammation and Ageing.
How to apply
Please click on the institution website where you will be taken to the MRC AIM website which contains full information as well as the application forms. Please ensure your application is submitted by the deadline of midday (GMT) Friday 12 January 2024 as late applications will not be considered.