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Investigating the role of centromeric RNA transcripts for centromere function and inheritance

  • Full or part time
    Dr P. Heun
    Dr Alastair Kerr
  • Application Deadline
    Sunday, January 05, 2020
  • Competition Funded PhD Project (Students Worldwide)
    Competition Funded PhD Project (Students Worldwide)

Project Description

The centromere is a unique chromatin domain important for proper segregation of chromosomes during mitosis. In most organisms, the position of the centromere is determined epigenetically by the centromere-specific incorporation of the H3-variant CENP-A Transcription at centromeres has been linked to the deposition of new CENP-A, although it is unclear whether transcription or the produced RNA are equally important. We and others have recently found that several components of the transcriptional machinery including RNA Polymerase II (RNAPII) and centromere-associated RNA transcripts temporally coincides with CENP-A loading in mitosis to G1. While we have evidence cells that the transcriptional process itself is required for CENP-A deposition at the centromere, this does not rule out a role for the centromeric transcripts themselves. To investigate this further we will purify nascent centromeric RNA transcripts, match them to the underlying centromeric DNA and identify potential binding partners. We will then aim to dissect the roles of transcription and RNA transcripts by specifically targeting transcriptional repressors or RNases to centromeric loci and investigate centromere inheritance and function. This work will be performed using Drosophila tissue culture cells as an ideal model system for simple centromeres.

http://www.wcb.ed.ac.uk/research/patrick-heun

Funding Notes

The “Visit Website” button on this page will take you to our Online Application checklist. Please complete each step and download the checklist which will provide a list of funding options and guide you through the application process.

If you would like us to consider you for one of our scholarships you must apply by 5 January 2020 at the latest.

References

1. McKinley and Cheeseman IM, The molecular basis for centromere identity and function. Nat Rev Mol Cell Biol. 2016, 17:16-29 Review
2. Bobkov G, Gilbert N and Heun P, Centromere transcription allows CENP-A to transit from chromatin association to stable incorporation. J Cell Biol 2018, 217: 1957-1972
3. Bobkov G, Axel Imhof and Heun P, Spt6 is a CENP-A maintenance factor, BioRxiv 2019

Related Subjects

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