About the Project
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a related area / subject. Candidates with a Bioscience background (e.g. physiology, biochemistry, pharmacology, biomedical sciences etc), or those with a background in Medicine wishing to do a laboratory based PhD are encouraged to apply.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit http://www.internationalphd.manchester.ac.uk
The project will involve a range of biochemical methods such as Western blotting, RT-qPCR, subcellular fractionation for mitochondrial isolation with functional analysis of mitochondrial function by measuring respiration rates (OCR) and Complex activity. Advanced 3-D electron microscopy imaging methods4,5 will be used to relate molecular and functional changes to mitochondrial remodelling. Proteomics and bioinformatic analyses coupled with biophysical techniques including microscale thermophoresis and isothermal titration calorimetry will be employed to delineate molecular mechanistic pathways to understand functional changes. The project will also employ cell culture, molecular biology methods including mammalian cell transfection techniques. Depending upon the interests and background of the student there may also be opportunities to undertake in-vivo studies.
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
2. Yu, E., Foote, K. and Bennett, M. Mitochondrial function in thoracic aortic aneurysms. Cardiovascular Research 2018; 114: 1696-1698
3. Jia, G, Hill, M.A. and Sowers, J.R. Diabetic Cardiomyopathy. Circulation Research 2018; 122:624-638
4. Pinali C. and Kitmitto A. Serial block face scanning electron microscopy for the study of cardiac muscle ultrastructure at nanoscale resolutions. Journal of Molecular and Cellular Cardiology 2014;76:1-11.
5. Pinali C. et al. Three-dimensional reconstruction of cardiac sarcoplasmic reticulum reveals a continuous network linking transverse-tubules: this organisation is perturbed in heart failure. Circulation Research 2013; 113: 1219-30
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