All the information to make every cell in the human body is encoded in the same 3 billion DNA letters of genetic code. So what allows each cell to be so different and make up all the tissues and organs in the human body? Cells use regulating proteins called transcription factors (TF) to switch on and off genes at different times and places in the body, creating a myriad of different cell types with different functions. When this goes wrong, it can lead to diseases such as cancer.
To work, TFs have to find the correct few letters of DNA in the right gene among the billions in the genome. How they do this is not fully understood. Using advanced microscopes, which can image individual TF molecules inside living cells, We showed that TFs find their binding sites by clustering together, allowing them to search more quickly by binding to multiple strands of DNA simultaneously. At the same time exciting new techniques are being developed which go beyond looking only at the sequence of DNA – A,T,C,G – but its 3D shape and mechanical properties, like flexibility. These techniques are opening up a whole new way to study how genes are switched on and off. This project will combine both techniques to try to understand how TF clusters alter the shape and flexibility of DNA and vice versa and what might go wrong in diseases such as cancer.
This project will provide experience and extensive training in single-molecule and super-resolution microscopy, genomic technologies, optics, image analysis and programming.
HOW TO APPLY
Applications should be made by emailing [Email Address Removed] with:
· a CV (including contact details of at least two academic (or other relevant) referees);
· a covering letter – clearly stating your first choice project, and optionally 2nd ranked project, as well as including whatever additional information you feel is pertinent to your application; you may wish to indicate, for example, why you are particularly interested in the selected project(s) and at the selected University;
· copies of your relevant undergraduate degree transcripts and certificates;
· a copy of your IELTS or TOEFL English language certificate (where required);
· a copy of your passport (photo page).
A GUIDE TO THE FORMAT REQUIRED FOR THE APPLICATION DOCUMENTS IS AVAILABLE AT https://www.nld-dtp.org.uk/how-apply. Applications not meeting these criteria may be rejected.
In addition to the above items, please email a completed copy of the Additional Details Form (as a Word document) to [Email Address Removed]. A blank copy of this form can be found at: https://www.nld-dtp.org.uk/how-apply.
Informal enquiries may be made to [Email Address Removed]
The deadline for all applications is 12noon on Monday 9th January 2023.
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