Lipid droplets are important intracellular particles that comprise neutral lipids surrounded by a phospholipid monolayer. They are a major reservoir for lipids that are then provided for energy, membrane biosynthesis and for signalling intermediates. Perilipin2 (PLIN2) associates with lipid droplets promoting their formation and maintenance. PLIN2 exhibits reduced inflammatory induced expression in monocytes from patients with Alzheimer’s disease vs healthy controls. Furthermore, microglia (brain macrophages) accumulate lipid droplets during ageing in mice and humans and lipid-droplet-accumulating microglia are functionally defective in relation to phagocytosis (bacterial killing) and generate higher levels of pro-inflammatory mediators.
We hypothesize that regulation of lipid droplets by PLIN2 alters macrophage homeostatic and inflammatory responses, and cellular metabolism, altering the risk of diseases of aging. To test this, we have generated a mouse model of enhanced PLIN2 expression in microglia. In vivo context is critical for physiologically-relevant macrophage function and this study will focus on in brain macrophages during aging.
The student will be embedded in the well-funded UK MRC Dementia Research Institute Centre at Cardiff and will work across two research groups with a long history of collaboration. They will benefit from expertise in macrophage biology (Phil Taylor) and lipid biochemisty (Valerie O’Donnell).
Entry Requirements
You will hold or expect to achieve a First or Upper Second Class degree in immunology, biochemistry, cell biology. As this is a training doctorate, previous research experience is not essential.
Applicants whose first language is not English are normally expected to meet the minimum University requirements (e.g. 6.5 IELTS).
How to Apply
This studentship has a start date of October 2023. In order to be considered you must submit a formal application via Cardiff University’s online application service.
There is a box at the top right of the page labelled ‘Apply’, please ensure you select the correct ‘Qualification’ (Doctor of Philosophy), the correct ‘Mode of Study’ (Full Time) and the correct ‘Start Date’ (October 2023). This will take you to the application portal.
In order to be considered candidates must submit the following information:
• Supporting statement
• CV
• Qualification certificates
• References x 2
• Proof of English language (if applicable)
International applicants are welcomed if the difference in fees can be covered.