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Making B-lymphocytes form pluripotent stem cells. pervisor


Project Description

B lymphocytes are a critical arm of the adaptive immune system. The in vitro generation of B cells from human pluripotent stem cells provides an opportunity to study normal immune development, to model disease and to generate cells and products for potential therapeutic use. We have methods for making definitive human hematopoietic lineages from PSCs, enabling us to generate both B and T lymphocyte progenitors in vitro.

This project will involve generating B cells from induced pluripotent stem cells using in vitro and in vivo models. Students will have access to established reagents, such as genetically modified PSC reporter lines, and highly evolved pluripotent stem cell differentiation protocols. Students will learn how to grow, genetically modify and differentiate pluripotent stem cells. The project will also involve techniques such as flow cytometry, immunofluorescence, confocal microscopy, and quantitative PCR. Depending on the direction of the work, generation and analysis of RNAseq data may also be necessary.

The Immune Development Laboratory is physically located at the Murdoch Children’s Research Institute, part of the Melbourne Children’s Campus, which also includes the Royal Children’s Hospital and University of Melbourne: See our website for further information.

https://www.mcri.edu.au/users/professor-ed-stanley.

*To receive a reply to your inquiry, you should:
* Proved a 3 sentence paragraph outlining why you are interested in B-cell development
* Provide a single question that they would like to answer regarding this cell type.
* Provide a copy of your CV
* Provide a copy of your academic transcripts

Funding Notes

Students enrol through the Department of Paediatrics, University of Melbourne. Scholarships and Fee Waivers are available from the University on a competitive basis (View Website). Successful students usually require a GPA of 85/100 in order to be competitive. English language requirements apply. Specifically, candidates need to have IELTS score of 7 overall with no individual task score being lower than 7.

*To receive a reply to your inquiry, you should provide:
* a 3 sentence paragraph outlining why you are interested in B-cell development .
* a copy of your CV
* a copy of your academic transcripts.

References

Publications can be most easily seen though my publicly accessible Google Scholar profile.

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