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Mass spectrometric imaging of biological tissues

  • Full or part time
  • Application Deadline
    Thursday, February 20, 2020
  • Funded PhD Project (Students Worldwide)
    Funded PhD Project (Students Worldwide)

Project Description

Mass spectrometry imaging (MSI) exploits the ability of mass spectrometry to identify molecules according to their specific mass and image their distribution across the sample. The method allows direct visualization of endogenous compounds (lipids, proteins, small molecules) as well as exogenous drugs and their metabolites. The aim of the research will be development of a suitable matrix-assisted laser desorption/ionization (MALDI) MSI method for the study of distribution and the effect of chosen drug selected tissues and cell lines. Special attention will be devoted to sample preparation and matrix deposition (spray, sublimation, piezoelectric dispenser) and data processing. The samples will be supplied by cooperating institutions (e.g. the Department of Experimental Biology, the Department of Pharmacology or the Regional Centre for Applied Molecular Oncology). The analyzed compounds include selected drugs or endogenous compounds (e.g. lipids, glycans). Hands-on experience with MALDI MS and software skills (MATLAB, R) are welcomed.

Keywords: mass spectrometry imaging, MALDI, MSI

HOW TO APPLY: Register for this call using the registration form at http://ls-phd.ceitec.cz/http-ls-phd-ceitec-cz/ and submit required documents to receive support with the preparation of the application and all formalities. Your application package will be forwarded to the supervisor for preliminary revision.
Applicants who wish to pursue a degree at the CEITEC PhD program must hold the equivalent of a Master’s degree (MSc) in a similar field (four- or five-year undergraduate degree). The application can be submitted before obtaining the Master’s degree; however, the applicant should obtain a degree within five months after the application deadline.

Funding Notes

Admission for studies, student registration for the full-time study program, and proper fulfillment of student duties constitute the student’s right to a regular income of 22 000 CZK (850 EUR). Information is available at View Website

Living costs and other practicalities available at View Website

References

1. Machálková, M.; Pavlatovská, B.; Michálek, J.; Pruška, A.; Štěpka, K.; Nečasová, T.; Radaszkiewicz, K..; Kozubek, M.; Šmarda, J.; Preisler, J.; Navrátilová, J. "Drug Penetration Analysis in 3D Cell Cultures Using Fiducial-Based Semi-automatic Coregistration of MALDI MSI and Immunofluorescent Images" Anal Chem 2019, in press. https://pubs.acs.org/doi/10.1021/acs.analchem.9b02462
2. Bednařík, A.; Machálková, M.; Moskovets, E.; Coufalíková, K.; Krásenský, P.; Houška, P.;, Kroupa, J.; Navrátilová, J.; Šmarda, J.; Preisler, J. "MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second" J Amer Soc Mass Spectrom 30, 2019, 289-298. http://doi.org/10.1007/s13361-018-2078-8

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