About the Project
The objectives of this project are to understand the molecular complexes that regulate cilia biogenesis and signalling, and to understand how their misregulation can lead to disease. We will focus on the regulation of centriole distal appendage proteins (DAPs).
We will use CRISPR/Cas9-engineered isogenic cell lines deficient in specific centriolar proteins, including DAPs, to understand how they are recruited to centrioles and how they regulate ciliogenesis. To this end, we will use various biochemical, cell biology and microscopy techniques. We will have access to animal models in collaboration with a number of labs at Brunel. At the end of the project the student will have developed tools to carry out research independently, and have substantial practical and conceptual knowledge of the cilia field.
T. Tony Yang, Weng Man Chong, Won-Jing Wang, Gregory Mazo, Barbara Tanos, Zhengmin Chen, Thi Minh Nguyet Tran, Yi-De Chen, Rueyhung Roc Weng, Chia-En Huang, Wann-Neng Jane, Meng-Fu Bryan Tsou, Jung-Chi Liao. Architecture of mammalian centriole distal appendages supports a matrix that gates the primary cilium. 2018. Nature Communications. 2018 May 22;9(1):2023. doi: 10.1038/s41467-018-04469-1. doi: https://doi.org/10.1101/193474
Failler M, Gee HY, Krug P, Joo K, Halbritter J, Belkacem L, Filhol E, Porath JD, Braun DA, Schueler M, Frigo A, Alibeu O, Masson C, Brochard K, Hurault de Ligny B, Novo R, Pietrement C, Kayserili H, Salomon R, Gubler MC, Otto EA, Antignac C, Kim J, Benmerah A, Hildebrandt F, Saunier S. Mutations of CEP83 cause infantile nephronophthisis and intellectual disability. Am J Hum Genet. 2014 Jun 5;94(6):905-14. doi: 10.1016/j.ajhg.2014.05.002. Epub 2014 May 29. PMID: 24882706.
Adly N, Alhashem A, Ammari A, Alkuraya FS. Ciliary genes TBC1D32/C6orf170 and SCLT1 are mutated in patients with OFD type IX. Hum Mutat. 2014 Jan;35(1):36-40. doi: 10.1002/humu.22477.
Chien-Hui Lo, I-Hsuan Lin, T. Tony Yang, Yen-Chun Huang, Barbara E. Tanos, Yeou-Guang Tsay, Jung-Chi Liao and Won-Jing Wang. TTBK2 phosphorylates CEP83 in promoting cilia initiation. 2019. Journal of Cell Biology. 2019 Aug 27. PMID:31455668
Andrew D. Jenks, Simon Vyse, Jocelyn P. Wong, Deborah Keller, Tom Burgoyne, Amelia Shoemark, Maike de la Roche, Martin Michaelis, Jindrich Cinatl, Paul H. Huang and Barbara E. Tanos. Primary cilia mediate diverse kinase inhibitor resistance mechanisms in cancer. 2018. 2018. Cell Reports. 2018 Jun 5;23(10):3042-3055. doi: 10.1016/j.celrep.2018.05.016.
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.