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Mechanisms of toxicity of metal nanoparticles in stem cells


research

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Dr Pavel Rossner No more applications being accepted Funded PhD Project (Students Worldwide)
Prague Czech Republic Biotechnology Cell Biology Molecular Biology Nanotechnology

About the Project

Department of Nanotoxicology and Molecular Epidemiology: http://iem.cas.cz/en/research/departments/department-of-nanotoxicology-and-molecular-epidemiology/

Although nanoparticles (NP) are widely used in numerous industrial and medical products, there is still limited knowledge on their potential impact on human health. Experimental studies indicate that NP may cause damage to genetic material and negatively affect cellular functions, although the biological effects depend on their physico-chemical characteristics. Stem cells that play an important role in regeneration and wound healing may be particularly sensitive to the deleterious effects of NP. The aim of the project is to investigate the impacts of metal NP with antimicrobial properties, on the processes and function characteristics of mouse mesenchymal stem cells (MSC). Among other parameters, the induction of DNA damage, oxidative stress, apoptosis, cell cycle changes, as well as global mRNA and miRNA expression, will be investigated in MSC treated in vitro with Ag, CuO and ZnO NP. The results will be further evaluated in vivo in mouse experimental models, in which the effects of NP on the processes of wound healing will be investigated.

The ideal candidate should:

- have a strong backgound in molecular biology and toxicology, and be familiar with the laboratory techniques used in these fields (e.g. cell cultivation, analyses of cytotoxicity, flow cytometry, comet assay, cytogenetic methods - micronuclei detection, analyses of reactive oxygen species and other parameters of oxidative stress, RT-PCR, gene expression analyses by next generation sequencing).

- speak fluent English

- be experienced in reading scientific literature and writing manuscripts

 How to apply: www.iem.cas.cz/phd

If you are interested, please visit the official website and submit the simple online application form. You can apply for up to 3 PhD positions. We are looking forward to hearing from you!


Funding Notes

After admission to the graduate program, the student usually receives an academic fellowship. In addition, the student becomes an IEM CAS employee and receives a salary (including payments towards social and healthcare insurance) and other benefits. This is financed by internal and grant funds of the IEM CAS.

References

1. Magdolenova, Z.; Collins, A.; Kumar, A.; Dhawan, A.; Stone, V.; Dusinska, M. Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology 2014, 8, 233–278, doi:10.3109/17435390.2013.773464.
2. Oyarzun-Ampuero, F.; Vidal, A.; Concha, M.; Morales, J.; Orellana, S.; Moreno-Villoslada, I. Nanoparticles for the Treatment of Wounds. Curr. Pharm. Des. 2015, 21, 4329–4341.
3. Vitale, I.; Manic, G.; De Maria, R.; Kroemer, G.; Galluzzi, L. DNA Damage in Stem Cells. Molecular Cell 2017, 66, 306–319, doi:10.
4. Abdal Dayem, A.; Lee, S.; Cho, S.-G. The Impact of Metallic Nanoparticles on Stem Cell Proliferation and Differentiation. Nanomaterials 2018, 8, 761, doi:10.3390/nano8100761.
5. Poetsch, A.R. The genomics of oxidative DNA damage, repair, and resulting mutagenesis. Computational and Structural Biotechnology Journal 2020, 18, 207–219, doi:10.1016/j.csbj.2019.12.013.
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