About the Project
Although we have a basic understanding of the membrane trafficking steps required for cell migration, we know very little about which motors drive particular steps, and whether specific motor subtypes have distinct roles. We will disrupt microtubule motor function using a combination of depletion (using siRNA and CRISPR/Cas9) and overexpression or microinjection of function-blocking motor protein domains. We will determine the effects these treatments have on cell migration in 2D and 3D, analysing the speed of movement and the ability of the cell to migrate in a defined direction. We will assess the trafficking of specific integrins, and test whether the organisation of the recycling endocytic pathway is changed. By imaging the dynamics of the membranes containing integrins with high temporal and spatial resolution in migrating cells, we will define how this organelle motility contributes to cell migration.
Training/techniques to be provided:
Cell culture of human cell lines; transient transfection and generation of stable cell lines; molecular biology techniques; RNAi; immunofluorescence microscopy; live cell imaging (phase constrast, fluorescence); 3D cell migration assays.
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a biological sciences subject. Candidates with experience in cell biology or with an interest in advanced light microscopy are encouraged to apply.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit www.internationalphd.manchester.ac.uk
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
Ruane, P. T., Gumy, L. F., Bola, B., Anderson, B., Wozniak, M. J., Hoogenraad, C. C., & Allan, V. J. (2016). Tumour Suppressor Adenomatous Polyposis Coli (APC) localisation is regulated by both Kinesin-1 and Kinesin-2. Scientific Reports, 6, 27456
Wilson BJ, Allen JL, Caswell PT. (2018) Vesicle trafficking pathways that direct cell migration in 3D and in vivo. Traffic, 19:899-909
Caswell PT, Zech T. (2018) Actin-Based Cell Protrusion in a 3D Matrix. Trends Cell Biol. 2018, 28:823-834
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