Looking to list your PhD opportunities? Log in here.
This project is no longer listed on FindAPhD.com and may not be available.
Click here to search FindAPhD.com for PhD studentship opportunitiesAbout the Project
Electron and captured energy flow within proteins is essential to life; these phenomena underpin cellular respiration and photosynthesis, both of which are dependent on complex protein machinery that support chains of redox active cofactors or chromophores. Despite the apparent complexity of these proteins and multi-protein assemblies, simple engineering rules are evident and can inform the design and construction of completely artificial proteins exhibiting sophisticated functions intrinsic to the respiratory and photosynthetic protein machinery. For instance, by minimising intermolecular distances between cofactors or chromophores, highly efficient and rapid energy and electron transfer can potentially be achieved in a de novo protein scaffold. The natural proteins and enzymes are also clearly modular in design, and this can also inform the construction of de novo proteins that can transfer electrons for tens of nanometers, and deliver captured light energy to photosynthetic reaction centres. It is therefore the goal of the laboratory to design and construct de novo proteins equipped with chains of redox-active cofactors and chromophores to gain both a deeper understanding of of processes fundamental to life, while providing a framework for exploiting such exceptional properties as efficient photon capture and energy transduction, long range and directional electron transfer, multi-electron redox catalysis, and transmembrane proton translocation.
The aims of this project are to use powerful computational and iterative approaches to the de novo design of multi-heme, and subsequently multi-chromophore, proteins. We will employ a combination of the Rosetta protein design package and atomistic biomolecular simulations, alongside electrostatic calculations, thereby facilitating an unparalleled control and precision over the protein sequence, structure and biophysical characteristics. This will enable directionality to be imprinted on the electron transfer through the proteins, a feat which has yet to be achieved. Given the similarity between heme and photosynthetic pigments, we will adapt our designs to accommodate multiple chromophores within the binding sites, thus creating soluble light harvesting units to study energy capture and transfer. We also aim to implement electrostatic calculations within our VR design framework which already includes the Rosetta protein design package. All designed proteins will be subjected to an array of biophysical techniques and spectroscopies (e.g. CD and ultrafast multidimensional pump-probe spectroscopy, electrochemistry), and where appropriate, directed evolution will be employed to improve characteristics such as cofactor binding.
Keywords: Computational de novo protein design; light harvesting; artificial photosynthesis; protein electron transfer; nanoscale wires

Search suggestions
Based on your current searches we recommend the following search filters.
Check out our other PhDs in Bristol, United Kingdom
Check out our other PhDs in United Kingdom
Start a New search with our database of over 4,000 PhDs

PhD suggestions
Based on your current search criteria we thought you might be interested in these.
Design, construction and testing of recombinant protein production systems for industrial biotechnology applications.
Aston University
Molecular Design for the Energy Harvesting Materials
The University of Manchester
Design and Engineering of Light-activated Enzymes for Chemicals Production
The University of Manchester