Tips on how to manage your PhD stipend FIND OUT MORE
University of Liverpool Featured PhD Programmes
University of Sussex Featured PhD Programmes
Queen’s University Belfast Featured PhD Programmes

Molecular Mechanisms of Receptor and Ion Channel Regulation in Pain and Inflammation


School of Pharmacy

About the Project

"Receptors and ion channels are responsible for the transmission of signals across cellular membranes that regulate a plethora of biological and cellular functions. The molecular mechanisms that regulate receptors and ion channels and their downstream signaling pathways in the cardiovascular and nervous systems are complex. Whereas receptors and ion channels were originally thought to act as single entities, it is now clear that protein•protein interactions, protein trafficking and sequestration and receptor co-operativity expand the repertoire of signaling cascades that can be elicited by a single agonist. The phenomenon of biased agonism (sometimes called ‘functional selectivity’) also adds further to this complexity. Our work is primarily focused on G protein-coupled receptors, toll-like receptors and ion channels involved in the transmission of pain and inflammatory processes and investigates how molecular and cellular mechanisms regulate signal transduction and contribute to normal physiological processes and promote disease.
Studies will provide will provide specialist training in state-of-the-art molecular, biochemical, pharmacological, microscopic and genetic tools to discover new targets for the treatment of pain and inflammatory processes that can be associated with many human disorders including migraine, asthma and inflammatory bowel disease.

• Methods include:
o Cell culture of cell lines
o Plasmid transfections, viral transductions
o Immunocytochemistry/immunohistochemistry
o Western blotting
o Confocal laser scanning microscopy
o FRET/BRET-based signaling assays
o Ca2+-signaling assays
o Plasmid DNA cloning, RT-PCR, qPCR
o Gene editing e.g., CRISPR/Cas9
o Cell-based assays e.g. viability and proliferation assays
o Gene reporter assays e.g., Luciferase assays

Key words: GPCRs, Ion channels, Signaling, Trafficking, Pain, Inflammation.

"


Funding Notes

Students with their own financial support are welcome to contact us any time. Information regarding the tuition fees for postgraduate research students can be found at the webpage linked below: View Website

References

"https://www.ncbi.nlm.nih.gov/pubmed/30804245
https://www.ncbi.nlm.nih.gov/pubmed/30181139
https://www.ncbi.nlm.nih.gov/pubmed/29394279
https://www.ncbi.nlm.nih.gov/pubmed/28959041

https://www.reading.ac.uk/pharmacy/about/staff/g-s-cottrell.aspx


Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here

The information you submit to University of Reading will only be used by them or their data partners to deal with your enquiry, according to their privacy notice. For more information on how we use and store your data, please read our privacy statement.

* required field

Your enquiry has been emailed successfully



Search Suggestions

Search Suggestions

Based on your current searches we recommend the following search filters.



FindAPhD. Copyright 2005-2021
All rights reserved.