About the Project
Studies will provide will provide specialist training in state-of-the-art molecular, biochemical, pharmacological, microscopic and genetic tools to discover new targets for the treatment of pain and inflammatory processes that can be associated with many human disorders including migraine, asthma and inflammatory bowel disease.
• Methods include:
o Cell culture of cell lines
o Plasmid transfections, viral transductions
o Western blotting
o Confocal laser scanning microscopy
o FRET/BRET-based signaling assays
o Ca2+-signaling assays
o Plasmid DNA cloning, RT-PCR, qPCR
o Gene editing e.g., CRISPR/Cas9
o Cell-based assays e.g. viability and proliferation assays
o Gene reporter assays e.g., Luciferase assays
Key words: GPCRs, Ion channels, Signaling, Trafficking, Pain, Inflammation.
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