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Lysosomes are membrane bound organelles characterised by a low pH, which contain hydrolytic enzymes that function as part of the cellular recycling system. The low pH associated with these organelles provides a unique opportunity for accumulation, release, and sensing using small molecules, permitting an understanding of this chemical environment and its relationship to disease. We will design, synthesise, and monitor small molecules that are able to examine each of these areas using bioinformatics to process and interpret the imaging data generated.
Key Words: chemical biology, synthesis, organic, probe, Raman Spectroscopy, bioinformatics
References
Ratiometric sensing of fluoride ions using Raman spectroscopy. Tipping, William, J.; Wilson, Liam T; Balieso, Sonja; Tomkinson, Nicholas, C. O.; Faulds, Karen, Graham, Duncan. Chem. Commun. 2020, 56, 14463–14466.

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