Dynein and kinesin are families of motor proteins that use ATP hydrolysis to transport cellular components and signalling molecules along the microtubule cytoskeleton. Dynein and kinesin also have critical roles in assembling larger cellular structures, such as the mitotic spindle, cilia and flagella. Our current goals are to understand how dynein and kinesin work together to form bidirectional transport systems [1,2] and how the motors selectively attach to cargoes . We are studying these questions using transport within cilia and flagella as a biomedically important model . Defective transport in cilia causes a variety of human disorders associated with vision impairment, skeletal abnormalities, cystic kidneys, and infertility, among other conditions. In this project, you will learn cryo-electron microscopy (cryo-EM), purification of multi-protein complexes, and cell biology techniques to advance understanding of motor proteins in health and disease.