**MRes Application of Biosurfactants as anti-biofilm agents

The global rise of antimicrobial resistance has potentially devastating consequences, particularly within the healthcare sector, and requires urgent attention. Research has focused on the search for, and development of, novel antimicrobial agents. However, commercialisation of new drugs is a lengthy process. Targeting surface biofilms, which contribute to pathogenicity and act as a reservoir of antibiotic resistant microorganisms, is another approach to the problem and reduces the likelihood that resistance will develop.
Biofilms also present a challenge however because they can withstand unfavourable environmental conditions and inhibitory substances such as biocides. They are therefore difficult to prevent and eradicate and conventional treatments are often ineffective. Surfaces in the healthcare environment, including devices such as catheters, may be colonised, and affected patients are susceptible to highly problematic, recurring infection.

Many microorganisms synthesise surface-active molecules called biosurfactants, some of which exhibit effective anti-microbial and/or anti-biofilm properties. The biocompatibility, low toxicity and biodegradability of these substances make them promising candidates for use in the clinical environment where they could be used treat and prevent biofilms.

The aim of this project is to establish the potential of actinomycete-derived biosurfactants for effective prevention and/or removal of clinical biofilms associated with intra-urinary devices. A range of techniques will be employed, including bacterial culture, biosurfactant extraction and purification, antimicrobial and anti-biofilm assays, and molecular identification of biosurfactant-producing actinomycetes. An innovative intra-urinary device model will also be established for further testing.

For more information, follow the link provided to Edinburgh Napier University’s research degrees application process and scroll down to the ‘Masters by Research’ (MRes) section. Here you will find more information about the application process. Please apply to the School of Applied Sciences.

In all correspondence, please quote project title and code: SAS0027


Academic qualifications
A first degree (at least a 2.1) ideally in Microbiology or a related biological science discipline with a good fundamental knowledge of Microbiology.

English language requirement
IELTS score must be at least 6.5 (with not less than 6.0 in each of the four components). Other, equivalent qualifications will be accepted. Full details of the University’s policy are available online.

Essential attributes:
• Experience of fundamental microbiology laboratory skills such as aseptic technique
• Competent in data analysis
• Knowledge of basic molecular biology techniques such as PCR
• Good written and oral communication skills
• Strong motivation, with evidence of independent research skills relevant to the project
• Good time management

Desirable attributes:
Practical experience of antimicrobial and/or anti-biofilm assays
Practical experience of basic molecular biology techniques such as DNA isolation and PCR