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Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN)


About This PhD Project

Project Description

A 4 years PhD position will be available in the group of Prof. Francesca Bottanelli at Freie Universität Berlin (Institute of Chemistry and Biochemistry) pending approval of the upcoming Collaborative Research Centre 958 "Scaffolding of Membranes - Molecular Mechanisms and Cellular Functions".

Job description:
In the project “Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN)” we will study the mechanisms of sorting at the TGN in epithelial cells by combining super-resolution stimulated emission depletion (STED) imaging, acute inactivation strategies and proximity-labelling based proteomics approaches. Specifically, we will investigate the contribution of membrane scaffolds in post-Golgi sorting and trafficking. Additionally, the candidate is expected to assist with teaching (seminars and practical courses).

Requirements:

University degree (Master) in Cell Biology, Biochemistry, Biophysics or related subjects.

Desirable:
Candidates are expected to have a strong interest in the investigation of intracellular trafficking and imaging. Motivation, ability to work in a collaborative environment and communication skills (in English) are essential. Prior experience in mammalian cell culture, molecular biology and imaging is desirable.



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