About the Project
The aim of one project, for example, is to examine which proline residues are critical for forming the correct structure of the neurotransmitter binding site. You will do this using site-directed mutagenesis to change each proline residue to alanine and then examine the effect of these changes on radioligand binding characteristics, function as assessed using electrophysiology and calcium imaging techniques, and localization of the receptor using immunocytochemistry.
Spier AD & Lummis, SCR (2000) The role of tryptophan residues in the 5-hydroxytryptamine3 receptor ligand binding domain. J Biol Chem, 275, 2650-5
Reeves, D.C., Goren, E.N., Akabas, M.H. & Lummis, S.C.R. (2001) Structural and electrostatic properties of the 5-HT3 receptor pore revealed by substituted cysteine accessibility mutagenesis. J. Biol Chem. 276, 42035-42042.
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