Aberdeen University Featured PhD Programmes
University of West London Featured PhD Programmes
Ulster University Featured PhD Programmes
University of Kent Featured PhD Programmes
University of Reading Featured PhD Programmes

Next generation infra-red glucose biosensors


Project Description

Glucose is a key metabolite for microorganisms, the most important energy source in more complex organisms and the key product of photosynthesis in plants. It is therefore crucial across the biological sciences to be able to accurately map glucose concentrations in living organisms and cells. The technology powering blood glucose monitoring devices used routinely in hospitals is electro-chemical, requiring invasive physical sampling. New sensor technology is in development which uses light to measure glucose concentrations. It works using a fluorescent molecular probe which binds glucose and changes the colour of fluorescently emitted light. These biosensors are entirely made of protein so can even be expressed natively in the organism. The problem is that currently these biosensors use visible wavelengths of light which are absorbed by tissues.

This project will develop the next generation of glucose biosensor using infra-red fluorophores. Light at these wavelengths passes through tissues and organisms much more easily and makes the biosensor technology compatible with many other commonly used optical microscopy techniques. Different infra-red fluorophores will be tested and a range of biosensors sensitive to different glucose concentrations will be developed to maximise applicability to different biological problems. Biosensors will be tested in test tubes but also in live cells, including model yeast and mammalian cell lines under the microscope. Training will be provided in molecular cloning, protein design production and purification, the design of biosensors, as well as microscopy and image analysis. This is a cutting-edge interdisciplinary project with potential for real impact in the biological sciences and also potential medical application in patient glucose monitoring.

HOW TO APPLY
Applications should be made by emailing with a CV (including contact details of at least two academic (or other relevant) referees), and a covering letter – clearly stating your first choice project, and optionally 2nd and 3rd ranked projects, as well as including whatever additional information you feel is pertinent to your application; you may wish to indicate, for example, why you are particularly interested in the selected project(s) and at the selected University. Applications not meeting these criteria will be rejected.
In addition to the CV and covering letter, please email a completed copy of the Additional Details Form (Word document) to . A blank copy of this form can be found at: https://www.nld-dtp.org.uk/how-apply.

Informal enquiries may be made to

Funding Notes

This is a 4 year BBSRC studentship under the Newcastle-Liverpool-Durham DTP. The successful applicant will receive research costs, tuition fees and stipend (£15,009 for 2019-20). The PhD will start in October 2020. Applicants should have, or be expecting to receive, a 2.1 Hons degree (or equivalent) in a relevant subject. EU candidates must have been resident in the UK for 3 years in order to receive full support. Please note, there are 2 stages to the application process.

References

High-speed imaging of glutamate release with genetically encoded sensors. Nature Protocols. 2019 May;14(5):1401-1424. doi:
10.1038/s41596-019-0143-9.

Single Synapse Indicators of Impaired Glutamate Clearance Derived from Fast iGlu u Imaging of
Cortical Afferents in the Striatum of Normal and Huntington (Q175) Mice. Journal of
Neuroscience. 2019 May 15;39(20):3970-3982. doi: 10.1523/JNEUROSCI.2865-18.2019.

Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. PNAS. 2018 May 22;115(21):5594-5599. doi: 10.1073/pnas.1720648115.

Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics. Scientific Reports. 2016 Dec 6;6:38276. doi: 10.1038/srep38276.

Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid Intracellular Calcium Dynamics. Scientific Reports. 2015 Nov 3;5:15978. doi:10.1038/srep15978.

A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochemical Journal. 2014 Dec 1;464(2):213-20. doi: 10.1042/BJ20141041.

Transport and self-organization across different length scales powered by motor proteins and programmed by DNA. Nature Nanotechnology, 2014, 9(1), 44–47.

Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae. Bio-protocol, 2018, 8(2).

Transcription factor clusters regulate genes in eukaryotic cells. ELife, 2017, 6, e27451.

Staphylococcus aureus toxin LukSF dissociates from its membrane receptor target to enable renewed ligand sequestration. The FASEB Journal, 2018, fj.201801910R-fj.201801910R

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2020
All rights reserved.