Faculty of Biology, Medicine and Health

The University of Manchester

  Ocular staining with lissamine green

  Dr P Morgan,  Applications accepted all year round  Self-Funded PhD Students Only

About the Project

This project will explore issues related to ocular staining with lissamine green. Although this staining agent has been used for many years in clinical ophthalmic practice, relatively little is known about its precise interaction with the ocular surface and an improved understanding in this area would improve clinical management and patient care.

The work will initially review current understanding of lissamine green staining via a literature view. Then, a series of clinical projects will be conducted to evaluate staining of the ocular surface and lid margins and how this changes diurnally both in contact lens wearers and non-wearers. The recovery from staining will also be assessed.

A project to identify the biological underpinnings of Lissamine Green staining will also be conducted. This work will involve impression cytology of the bulbar and lid margin conjunctiva and determining the nature of the removed cells using a range of cell biology techniques

Training/techniques to be provided

Training will be offered in: critical analysis of the literature, clinical study design, data analysis, oral and presentation skills, clinical and laboratory techniques.

Entry requirements

Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in optometry or a related subject area. The work would especially suit a GOC-registered optometrist and someone with experience in cell biology laboratory techniques.

Before you Apply

Applicants must make direct contact with preferred supervisors before applying. It is your responsibility to make arrangements to meet with potential supervisors, prior to submitting a formal online application.

How To Apply

For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (https://www.bmh.manchester.ac.uk/study/research/apply/). Informal enquiries may be made directly to the primary supervisor. On the online application form select PhD Optometry.

For international students, we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit https://www.bmh.manchester.ac.uk/study/research/programmes/integrated-teaching/

Your application form must be accompanied by a number of supporting documents by the advertised deadlines. Without all the required documents submitted at the time of application, your application will not be processed and we cannot accept responsibility for late or missed deadlines. Incomplete applications will not be considered.

If you have any queries regarding making an application please contact our admissions team .

Equality, Diversity and Inclusion

Equality, diversity and inclusion is fundamental to the success of The University of Manchester, and is at the heart of all of our activities. The full Equality, diversity and inclusion statement can be found on the website https://www.bmh.manchester.ac.uk/study/research/apply/equality-diversity-inclusion/

Medicine (26)

Funding Notes

Applications are invited from self-funded students. This project has a Band 2 fee. Details of our different fee bands can be found on our website View Website

References

Maldonado-Codina C., Read M.L., Efron N., Dobson C.B., Morgan P.B. Observation of solution-induced corneal staining with fluorescein, rose bengal and lissamine green (2013) Contact Lens and Anterior Eye, 36 (5), pp. 267 – 270 DOI: 10.1016/j.clae.2013.02.011
Efron N., Brennan N.A., Morgan P.B., Wilson T. Lid wiper epitheliopathy (2016) Progress in Retinal and Eye Research, 53, pp. 140 – 174 DOI: 10.1016/j.preteyeres.2016.04.004
Khan T.F., Price B.L., Morgan P.B., Maldonado-Codina C., Dobson C.B. Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment (2018) International Journal of Biochemistry and Cell Biology, 101, pp. 54 – 63 DOI: 10.1016/j.biocel.2018.05.01
Bakkar M.M., Hardaker L., March P., Morgan P.B., Maldonado-Codina C., Dobson C.B. The cellular basis for biocide-induced fluorescein hyperfluorescence in mammalian cell culture (2014) PLoS ONE, 9 (1), art. no. e84427 DOI: 10.1371/journal.pone.0084427
Navascues-Cornago M., Morgan P.B., Maldonado-Codina C. Lid margin sensitivity and staining in contact lens wear versus no lens wear (2015) Cornea, 34 (7), pp. 808 – 816 DOI: 10.1097/ICO.0000000000000448

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Faculty of Biology, Medicine and Health

Tackle real world challenges, make a difference, and elevate your career with postgraduate research in the Faculty of Biology, Medicine and Health at Manchester. From biochemistry to neuroscience, cancer sciences to medicine, audiology to mental health and everything in between, we offer a wide range of postgraduate research projects, programmes and funding which will allow you to immerse yourself in an area of research you’re passionate about.

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Ranked the best place to live in the UK (The Economist Global Liveability Index, 2022), Manchester boasts world-class culture, iconic sports, a thriving music and food scene, and much more. It's not just a place to research, it's a place to call home.

Experience PhD life as part of a diverse postgraduate research community of more than 1,000 postgraduate researchers at the 29th most international university in the world (Times Higher Education, 2023).

With 93% of research activity at the University rated as 'world-leading' or 'internationally excellent' (Research Excellence Framework, 2021), you'll get the chance to have an impact on global health and science challenges.

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About Faculty of Biology, Medicine and Health

At Manchester, postgraduate researchers are at the heart of our mission to tackle pressing global challenges in biological, medical and healthcare sciences - and you could be too.

By choosing Manchester for your postgraduate research, you’ll be joining a university with an exceptional research reputation, where 93% of research is world-leading or internationally excellent (REF, 2021) and where your work will have real-world impact.

You’ll research in world-class facilities alongside leading experts at the forefront of innovation, collaborating across disciplines to pioneer new treatments, advance scientific knowledge, and improve healthcare globally.

Supported by our dedicated Doctoral Academy and strong industry links, you'll experience PhD life in a vibrant, welcoming and diverse postgraduate research community.

And you’ll leave with the specialist knowledge, research experience and transferable skills that will shape your future in academia, research or industry.





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