Impact of platelet-derived extracellular vesicles on the quality and function of blood components in transfusion science practice
Blood and blood-derived products are a scare resource that are essential for the practice of modern medicine. It is essential to ensure that all donor blood is used as effectively as possible, to ensure the best clinical outcomes. Deleterious changes may occur during the storage of donor material, which contribute to sub-optimal clinical outcomes and waste of blood products. The content of extracellular vesicles – sub-micron membranous vesicles released from cells – can increase inside red cell blood packs during storage. Data on the changes in stored platelet packs – used to support blood coagulation in critical patients – is very limited.
This study will address this knowledge gap and define the physical, biochemical and functional changes that occur to platelet-derived extracellular vesicles (PEV) during the preparation, processing and storage of blood components. By combining metabolic, proteomic, functional and genetic analysis we will link the initial characteristics of donors to the development of storage-associated changes of clinical relevance (i.e. in PEV). This will identify early predictors of final product quality and identify routes to prevent these defects (e.g. using protective additives). The data from this project will be critical to develop improved processes and markers of component quality and so enhance transfusion practice.
The factors that influence the quality of blood components used in transfusion science are incompletely understood, leading to waste and sub-optimal clinical outcomes. This project will define the molecular changes that occur in platelet-pack associated extracellular vesicles to identify improved markers and a pathway to improved quality that reduces waste.
Student requirements for this project
Essential Criteria
· Level 8 Degree (BSc (Hons))in Medical Science or related discipline, with a minimum final award of a second class, upper (e.g. 2.1, 2h)
Desirable Criteria
· Eligible for CORU Medical Scientist Registration
- Research experience using human biological material
- Experience in working with blood components
· Completion of Transfusion Science and/or Haematology modules at undergraduate or postgraduate level.
Students are required to select and complete modules in the areas of transversal skills (20 ECTS) and discipline skills (20 ECTS) during the course of their study. Opportunities to maintain practice will also be provided. This position is a collaboration between the Irish Blood Transfusion Service and Technological University Dublin. This collaboration offers an excellent opportunity for a motivated person to bring together fundamental and practice-based research. It is of interest to those seeking a research-focused career in medical science.
If you are interested in submitting an application for this project, please complete an Expression of Interest (https://www.tudublin.ie/media/website/research/postgraduate-research/graduate-research-school/documents/Expression-of-Interest-form.docx)
and email it to [Email Address Removed].