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  PhD student position: deciphering the molecular basis of allostery in CRISPR associated proteins using single-molecule experiments


   Department of Chemistry

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  Prof Sonja Schmid  No more applications being accepted  Funded PhD Project (Students Worldwide)

About the Project

- Level of employment / possibly date of entry

100%, starting summer (negotiable)

 

- Intro

The Biomolecular Nano-Dynamics group at the Dept. of Chemistry, University of Basel is looking for a highly talented and motivated PhD student with a background in (bio-)physics, or biophysical chemistry to join our team for the study of allosteric mechanisms governing recently discovered CRISPR associated proteins using novel techniques, such as single-molecule fluorescence/FRET and nanopore trapping.

- Your position

This PhD project will grow with you. You will create time-resolved direct observations of single CARF proteins – aka. CRISPR suicide proteins – undergoing allosteric activation, substrate processing, deactivation, etc. How this works, on what timescales, by which conformational rearrangements has not been observed yet in real-time, leaving the underlying molecular processes obscure. In this PhD project, you’ll be able to solve (some of) these nanoscale puzzles. Steps involved in the project:

·      Single-molecule fluorescence/FRET/DyeCycling experiments

·      Solid-state nanopore/NEOtrap experiments

·      Advanced data analysis, coding, kinetic modeling

·      Protein production and functionalization

- Your profile

Prior experience in several of these areas is desired:

·      Single-molecule experiments and interpretation

·      Advanced data analysis, coding, modeling, machine learning

·      Optics and fluorescence microscopy and/or nanopore techniques

·      Protein biochemistry

Essential requirements:

·      MSc degree in physics or (bio-)chemistry with excellent grades

·      High motivation and curiosity about fundamental questions on the physical-chemistry of life

·      Independent analytical thinking and systematic work style

·      Excellent experimental problem-solving skills and perseverance

·      Friendly team player proficient in English communication (speaking & writing)

- We offer

·      An exciting and fully funded PhD project in Basel, Switzerland

·      Embedding in a productive, interdisciplinary group with an open and interactive lab culture

·      State-of-the-art research infrastructure and facilities

·      A highly international and interdisciplinary setting stimulating your professional and personal development

·      The GRACE graduate center, wide-ranging sports offers, etc.

·      A beautiful and very livable city at the intersection of Germany, France, and Switzerland

It goes without saying that we welcome applications of friendly and ambitious humans of all kind.

- Application/contact

Applications are invited before/by April 30th, 2024. Please submit your application via [Email Address Removed] with subject ‘Application PhD4 Firstname Lastname’, and include:

your letter of motivation, your CV, and email addresses of 2-3 references – all merged into one single pdf file namedApplication PhD4 Firstname Lastname.pdf’.

For questions, please contact Prof. Sonja Schmid: [Email Address Removed] .

Biological Sciences (4) Chemistry (6) Physics (29)

 About the Project