A fully-funded, BBSRC-supported PhD studentship position is available in the groups of Dr Marina Kuimova, Professor Ramon Vilar and Dr Vannier on a multidisciplinary project, based within the Department of Chemistry at the White City Campus of Imperial College London.
This project will focus on the development of novel probes and imaging techniques to monitor the formation of non-canonical DNA structures termed G-quadruplexes. Over the past few years, mounting experimental evidence suggested that these non-canonical DNA structures may well play essential biological roles. However, to date there is still little direct evidence that G-quadruplexes are functional in live cells. The PhD project will build on our ‘proof of concept’ study using Fluorescence Lifetime Imaging Microscopy (FLIM).
The successful applicant will be trained to perform cellular imaging including FLIM and spectroscopic characterisation of new fluorescent probes and their interaction with various DNA topologies. The main focus will then be to use these techniques to study G-quadruplex formation and its relationship to cell function. There is also scope to design and synthesise novel optical probes, provided the applicant has appropriate expertise and an aptitude for synthesis.
UK or EU nationals who are ordinary UK residents are eligible to apply. Candidates should have a BSc degree at 2.1 level or higher, or an equivalent qualification, to be considered for 1 year Masters + 3 years PhD training programme. Students with a relevant Masters, at Merit-level or better, will be considered for a +3 programme.