About the Project
We have identified a key regulatory step in plant sperm cell development that involves the Arabidopsis thaliana transcription factor DUO1. This protein is widely conserved in important food crops such as maize, wheat, rice and tomato and forms a regulatory module with its target protein DAZ1 (a zinc finger transcription factor) (Borg et al., 2014). An exciting recent discovery is that this module is ancient and is even needed for sperm development in early land plants, such as mosses and liverworts (Higo et al., 2018).
Project aims & description
This project will explore the conservation and mechanisms by which the DUO1-DAZ1 module coordinates cell proliferation with sperm differentiation. Studies of gene function will involve those in crop species including tomato, as well as in early land plant models. Genetic and molecular analyses will be combined with comparative transcriptome studies to uncover co-expression and co-function gene networks. This will help to discover novel plant fertility genes of potential value. Overall, the project seeks to establish how gene function and evolution has shaped the fertility of some of our important food crops, starting from their earliest origins.
The impact of the research is also expected in the sphere of novel plant breeding applications including applications such as hybrid seed production and the control of gene flow.
Year 1. Construct novel germline mutants using CRISPR-Cas9 technology and identify germline targets of the DUO1-DAZ1 regulon based on bioinformatic analysis of transcriptome data.
Year 2. Use genetic and molecular methods to analyse germline mutants and establish in vitro/in vivo DNA binding assays for DAZ1.
Year 3. Devise a network model for the contribution of DAZ1-targets to male germline development and analyse gene function by gene-editing and manipulation of protein function.
UK/EU fees and stipend at UKRI rates. For 2020 this will be £15,285 pa
Eligibility: UK/EU applicants only.
Applicants are required to hold/or expect to obtain a UK Bachelor Degree 2:1 or better in a relevant subject.
The University of Leicester English language requirements apply where applicable: https://le.ac.uk/study/research-degrees/entry-reqs/eng-lang-reqs/ielts-65
How to apply:
To apply for the PhD please refer to the guidelines and use the application link at https://le.ac.uk/study/research-degrees/funded-opportunities/bbsrc-mibtp
Please also submit your MIBTP notification form at https://warwick.ac.uk/fac/cross_fac/mibtp/pgstudy/phd_opportunities/application/
Project / Funding Enquiries: (Prof Dave Twell) [Email Address Removed]; [Email Address Removed]
Application enquiries to [Email Address Removed]
Borg, M., et al. (2014). An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis. Plant Cell 26:1-17.
Brownfield, L., et al. (2009) A plant germ cell-specific integrator of cell cycle progression and sperm specification PLoS Genetics 5: e1000430.
Berger, F. and Twell, D. (2011) Germline specification and function in plants. Annual Review of Plant Biology 62:461-484.
Higo et al., (2018) Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants. Nature Communications 9, 5283.
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