About the Project
Current diagnosis of fungal infections relies on the isolation and identification of fungi from clinical samples, but such microbiological methods are time consuming and lack sensitivity. Alternative fast, efficient and economic diagnostic methods and tools are urgently required. Nucleic Acids Enzymes are catalytic DNA or RNA sequences that can act as biomarker sensors and molecular switches. Innovative multi-component PlexZyme systems that can identify DNA biomarkers with high sensitivity and specificity have been recently developed. PlexZyme biosensors efficiently differentiate and quantify target biomarkers that differ in a single base pair thus allowing species-level identification of microorganisms and/or detection of single nucleotide polymorphisms associated with drug resistance. PlexZyme biosensors offer superior multiplexing capacity compared to alternative technologies and can be used under isothermal conditions or in cycling temperatures. They are relatively inexpensive, chemically stable and require simple instrumentation, making them ideal diagnostic products for use in resource constrained medical facilities and diagnostics laboratories worldwide.
This project will develop and implement PlexZymes as simple nucleic acids biosensors for the accurate, fast and reliable diagnosis of human candidiasis, Combining the use of molecular sensors for Candida DNA biomarkers with isothermal DNA amplification, we will create innovative diagnostic tools amenable to future automation that can be applied at the point of care.
Molecular diagnostics; fungal infections; human candidiasis; Candida; PlexZyme technology; molecular biosensors; Medical Mycology.
Schuetz AN. 2013. Invasive fungal infections: biomarkers and molecular approaches to diagnosis. Clinics in laboratory medicine 33:505-25
Brown GD, Denning DW, Gow NA, Levitz SM, Netea MG, White TC. 2012. Hidden killers: human fungal infections. Science translational medicine 4:165rv13
Enoch DA, Ludlam HA, Brown NM. 2006. Invasive fungal infections: a review of epidemiology and management options. Journal of medical microbiology 55:809-18
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