About the Project
The laboratory of Dr. Shih-Chieh Ti in the School of Biomedical Sciences at the University of Hong Kong (https://www.sbms.hku.hk/) seeks enthusiastic and self-motivated Ph.D. students to investigate the underlying mechanism for human tubulin isotypes to regulate the biological functions of microtubules.
The human genome encodes multiple α- and β-tubulin gene families (i.e., isotypes) that have the protein expression profile varying with cell types and developmental stages. While single point mutations in a specific tubulin isotype have been associated with human diseases, the molecular mechanism by which tubulin isotypes confer microtubules’ functions remains poorly understood. Using our field-leading strategy to generate isotypically pure recombinant human tubulin, Ti Lab will employ combinatorial approaches of biochemistry, biophysics, chemical biology, and structural biology to investigate the intrinsic properties of different human tubulin isotypes. Please see the following references to learn more about Dr. Ti’s previous work.
An ideal candidate should have excellent experiment and communication skills. Preference will be given to applicants with a strong background in molecular biology, protein purification, and cell culture. Please send (1) your CV, (2) a brief description of research interests and experience, and (3) three confidential letters of reference to [Email Address Removed].
Faculty information, funding opportunities and application deadlines: https://www.findaphd.com/phds/program/biomedical-research-hku-li-ka-shing-faculty-of-medicine/?i586p4119
References
1) Ti, S.C.*; Wieczorek, M.; Kapoor, T.M.* (2020) “Purification of affinity-tag free recombinant tubulin from insect cells” STAR Protocols, 1, 1, 10011
2) Wieczorek, M*; Urnavicius, L*; Ti, S.C., Molloy, K.R.; Chait, B.T.; Kapoor, T.M. (2020) “Asymmetric molecular architecture of the human γ-tubulin ring complex” Cell, 180, 165-175. PMID: 31862189.
3) Ti, S.C.; Alushin, G.M.; Kapoor, T.M. (2018) “Human β-tubulin isotypes can regulate microtubule protofilament number and stability” Developmental Cell, 47, 175-190. PMID: 30245156.
4) Pamula, M.C.; Ti, S.C.; Kapoor, T.M. (2016) “The structure core of human β tubulin confers isotype-specific polymerization properties” J. Cell Biology, 213, 425-33. PMID: 27185835.
5) Ti, S.C.; Pamula, M.C.; Howes, S.C.; Duellberg, C.; Cade, N.I.; Kleiner, R.E.; Forth, S.; Surrey, T.; Nogales, E.; Kapoor, T.M. (2016) “Mutations in human tubulin proximal to the kinesin-binding site alter dynamic instability at microtubule plus and minus ends” Developmental Cell, 37, 72-84. PMID: 27046833.
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