Nanoparticles have been intensively used for the delivery of drugs to target tissues such as the lymph nodes, brain, liver and tumors. However, the most commonly used nanoparticles require a high amount of carrier materials which hinders their ability to load high doses of drug, limiting their clinical applications. Nanocrystals, on the other hand, are nanoparticles formed only by the drug, which allows to deliver substantially higher drug payloads. In this project, the applicant will use top-down techniques to reduce the particle size of the drug and precisely engineer their surface properties in order to increase drug absorption in target tissues. The formulations will be fully characterised in vitro and successful candidates will be tested in cell cultures and animal models with the aim of evaluating their pharmacokinetics and biodistribution profiles. A strong emphasis will be placed in boosting both the theoretical and technical skills of the student, as well as enhancing their communication and teamwork abilities. All these aspects together will greatly improve the future professional perspective and employability of the candidate.