About the Project
While cell free systems have already been used to prototype a large number of gene circuits using transcriptional repressors, building functional activators has remained elusive, despite their fundamental role in living cells. This project plans to deploy a number of different approaches from in vivo activator engineering and programmable gene regulation  to build a repertoire of cell-free activators, forward-engineer a new class of gene circuits based on these parts, and understand the properties and limitations of these newly-engineered cell-free systems.
The ideal candidate will be a highly motivated biologist interested in quantitative approaches to gene circuit engineering, or a physicist/engineer interested in applying their skills to a wet-lab environment. Experience in any of the following areas is highly desirable: molecular biology, cell culture, microfluidics, microscopy, programming.
The project will be jointly supervised by Dr Nadanai Laohakunakorn (https://nadanai263.github.io/) and Dr Baojun Wang (http://wang.bio.ed.ac.uk/), within the School of Biological Sciences.
If you would like us to consider you for one of our scholarships you must apply by 5 January 2020 at the latest.
2. Liu, Y., Wan, X., and Wang, B. Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria. Nature communications 10:3693 (2019)
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