Quantum Dot Redox Sensing in Live Cells

The redox potential of cells (and their environments) is important for almost any process in the cell. Moreover, the redox balance in cancer cells is known to be different from that of healthy cells and thus it is becoming increasingly recogised as an important measurement for understanding processes in cells. In this project we will be exploring the use of quantum dots to be used to fluorescently sense the Redox potential inside individual cells and spheroids. The sensor is based on electron transfer with electron acceptor ligands which can quench the fluorecence when in the oxidised state. The quenching process is removed when the ligand is in the reduced state. This work builds on previous studies we have performed in the group. The project requires experimental work in a multidisciplinary environment. The quantum dots are highly flurescent and have tunable emission, making them ideal for this task.