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Regulation of CADM1 Alternative Splicing

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  • Full or part time
    Prof I C Eperon
    Prof P Bradding
    Dr C Dominguez
  • Application Deadline
    No more applications being accepted
  • Competition Funded PhD Project (European/UK Students Only)
    Competition Funded PhD Project (European/UK Students Only)

Project Description

CADM1 is a tumour suppressor that plays an important role in mediating adhesion between cells. It produces several isoforms by alternative splicing, and these appear to have quite distinct properties. In particular, an excess of one of these isoforms appears to play a significant role in the malignancy of lung mast cells. The purpose of this project is to determine how the relative levels of the isoforms are maintained in normal cells and how best to shift splicing in malignant cells so as to inhibit the spread of tumours.

The project will begin with a characterisation of the abundance of the different isoforms in various lung cell types, using bioinformatics, PCR and western blotting. Isoform-specific antibodies will be produced by phage display. To identify the sequences involved in regulating the appearance of the different isoforms, we will create mini genes and subject these to mutagenesis and transfection into cells; the isoforms expressed will be identified by RT-PCR. These sequences will then be targeted by modified oligonucleotides and we will study the effects of altered splicing on the behaviour of lung cancer cells. Further work might involve developing small molecule splicing modulators as a starting point for potential therapies.

Entry requirements:
You must hold (or be about to obtain) a First or an Upper Second Class UK undergraduate degree or an equivalent degree from a recognised EU institution, in an area relevant to the projects you are applying for.

Qualifications (or a combination of qualifications and experience) which demonstrate equivalent ability and attainment will also be considered.

For example, a less than sufficient first degree may be enhanced to meet the requirements by the acquisition of a Distinction at Masters level or significant research experience gained from employment).

Informal enquiries:
If you have any questions or would like to find out more about IMPACT, please get in touch.
Email us at [Email Address Removed]

Eligibility:
UK/EU applicants only

To apply:
Please refer to https://more.bham.ac.uk/mrc-impact/phd-opportunities/

Funding Notes

The Studentship provides 3.5 years of Stipend at UKRI rates and Tuition fees at UK/EU rates.



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