This project will address the overall aim of characterising macrophage heterogeneity in murine wounds from healthy and chronic wound models (diabetes and ageing).
The hypothesis is that healthy wounds will display less macrophage heterogeneity, as macrophages respond to environmental signals robustly and in a stereotypical fashion in healthy wounds. Chronic wounds would display increased heterogeneity as many macrophages do not interpret environmental signals appropriately.
The project will involve analysing and integrating RNA-seq and ChIP-seq data from macrophages stimulated with pro-inflammatory molecules. Selection of targets will be based on pathway analysis of the overlapping loci showing differential expression and differential transcription factor binding (by p65/RelA). The student will design RNA in situ probes for smiFISH and HCR FISH, and then image wound tissue sections from healthy, diabetic and aged mice, as well as cultured macrophages under different inflammatory conditions. Heterogeneity of gene expression under different inflammatory conditions will be measured using IMARIS software. Results will provide insights into macrophage regulation in healthy and chronic wounds.
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a related area/subject. Candidates with previous laboratory experience are particularly encouraged to apply.
How To Apply
For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (https://www.bmh.manchester.ac.uk/study/research/apply/). Informal enquiries may be made directly to the primary supervisor. On the online application form select the appropriate subject title.
For international students, we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences.
Equality, Diversity and Inclusion
Equality, diversity and inclusion is fundamental to the success of The University of Manchester, and is at the heart of all of our activities. The full Equality, diversity and inclusion statement can be found on the website https://www.bmh.manchester.ac.uk/study/research/apply/equality-diversity-inclusion/”