About the Project
We have recently shown how cohesin contributes to different aspects of meiotic chromosome function (3, 4, 5). The goal of the current project is to investigate the molecular mechanisms by which cohesin complexes differing in their kleisin subunit control the structure and function of mitotic and meiotic chromosomes in the nematode C. elegans. To achieve this, the project will combine functional in vivo studies, gene expression profiling, analysis of chromosome structure in 3D-intact nuclei by super-resolution microscopy, and single molecule approaches. This aspect of the project will involve a collaboration with the Aragon (Cell Cycle) and Rueda (Single molecule imaging) groups at the LMS, who recently developed methods to investigate the molecular activity of cohesin on DNA topology using optical tweezers (6). This project exploits the experimental advantages of C. elegans and state-of-the-art imaging and biophysical approaches to address a fundamental question of chromosome biology that is highly relevant to development and fertility in humans
To Apply: Please visit our website (https://lms.mrc.ac.uk/study-here/phd-studentships/lms-3-5yr-studentships/) to download an application form.
This project is direct funding available for UK/EU students only.
Ishiguro K-i (2019). The cohesin complex in mammalian meiosis. Genes Cells 24: 6.
Ferrandiz N, Barroso B, Telecan O, Shao N, Kim H-M, Testori S, Faull P, Cutillas P, Snijders AP, Colaiácovo MP and E. Martinez-Perez (2018). Spatiotemporal regulation of Aurora B recruitment ensures cohesion release during oocyte meiosis. Nat Commun 9: 838.
Crawley, O., Barroso, C., Testori, S., Ferrandiz, N., Silva, N., Castellano-Pozo, M., Jaso-Tamame, A.L., and Enrique Martinez-Perez (2016). Cohesin-interacting protein WAPL-1 regulates meiotic chromosome structure and cohesion by antagonizing specific cohesin complexes. eLife, 5: e10851.
Castellano-Pozo M, Pacheco S, Sioutas G, Jaso-Tamame AL, Dore MH, and E Martinez-Perez (2019). Reactivation of chromosome signalling induces reversal of the meiotic program. bioRxiv 754341.
Gutierrez-Escribano P, Newton MD, Llauro A, Huber J, Tanasie L, Davy J, Aly I, Aramayo R, Montoya A, Kramer H, Stigler J, Rueda D, and L Aragon (2019). A conserved activity for cohesin in bridging DNA molecules. bioRxiv 757286.
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