About the Project
We are interested in two classes of ribosome-associated proteins RNA-binding protein and GTP-binding “G proteins”. This project would study one of a number of interesting proteins. G proteins commonly act as molecular switches to regulate a wide-range of cellular events. Some G proteins such as eIF2 and eEF1A have well defined roles in protein synthesis (see references 1 and 5 below). Other G protein roles are much less clear or are not known, despite the fact that protein homologs exist in all forms of life.
This project will make use of modern molecular genetic tools to study how one or more G protein interacts with ribosomes and functions to modulate protein synthesis. These studies will make use of the wide variety of molecular techniques available using a yeast-based cell system which contains factors with clear human homologs and highly conserved translation mechanism with all other eukaryotes.
Candidates are expected to have, or about to obtain, an upper second class (or equivalent) undergraduate degree in a related subject area. A Masters qualification in a similar area would be an advantage.
2. Castelli LM Talavera D, Mohammad-Qureshi SS, Kershaw CJ, Costello JL, Rowe W, Sims PF, Ashe MP, Grant CM, Hubbard SJ, and Pavitt GD (2015). The 4E-BP Caf20p mediates both eIF4E-dependent and independent repression of translation. PLOS Genetics 11:e1005233
3. Costello JL, Rowe W, Castelli LM, Kershaw CJ, Talavera D, Sims PF, Grant CM, Pavitt GD, Hubbard SJ and Ashe MP (2015) Global mRNA selection mechanisms for translation initiation. Genome Biology 16 (1), 10.
4. Kershaw CJ Costello JL, Castelli LM, Talavera D, Rowe W, Sims PF, Ashe MP, Hubbard SJ, Pavitt GD and Grant CM (2015) The yeast La Related Protein Slf1p is a Key Activator of Translation During the Oxidative Stress Response. PLOS Genetics 11(1):e1004903.
5. Jennings MD, Zhou Y, Mohammad-Qureshi SS, Bennett D, Pavitt GD (2013) eIF2B promotes eIF5 dissociation from eIF2•GDP to facilitate guanine nucleotide exchange for translation initiation. Genes Dev 27:2696-707
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