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Replication and transcription machinery collisions in Escherichia coli.


Project Description

DNA duplication is essential for life but replication forks can be blocked when
they collide with proteins bound to DNA. Exactly how multiple rescue
pathways contribute to uninterrupted DNA replication is unknown and is a
problem shared by all life. In bacteria DNA replication occurs at the same
time as transcription of genes. Therefore the most common obstacles for
replication forks are transcribing RNA polymerases. DNA can be damaged
when the molecular machines responsible for reading and copying DNA
collide with each other. This in turn can cause genome instability and cell
death.

This project aims to determine the molecular mechanisms that reduce and
resolve conflict between the crucial biological processes of DNA replication
and transcription. We will use Escherichia coli as a model organism and
focus on helicases that promote the replication of transcribed DNA. Bacterial
replication forks can be reconstituted in a tube and we can use this to
biochemically investigate replication blocks and their consequences. This
project will also characterise the location and binding partners of proteins of
interest. The combination of biophysics, advanced imaging, biochemistry and
molecular biology will ensure a highly successful project with training in
pioneering techniques.

Funding Notes

This studentship is fully funded for four years and covers: (i) a tax-free annual stipend at the standard Research Council rate (£15,009 estimated for 2020 entry), (ii) research costs, and (iii) tuition fees at the UK/EU rate.

References

Entry requirements: Students with, or expecting to gain, at least an upper second class honours degree, or equivalent, are invited to apply. The interdisciplinary nature of this research project means that we welcome applications from students with backgrounds in any biological, chemical, and/or physical science, or students with mathematical backgrounds who are interested in using their skills in addressing biological questions.

How good is research at University of York in Biological Sciences?

FTE Category A staff submitted: 44.37

Research output data provided by the Research Excellence Framework (REF)

Click here to see the results for all UK universities

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