Self-Funded PhD Project: Electrochemistry at CYP51A1 Thin Films: a Novel Electrochemiluminescence (ECL)-based Approach to Evaluate Inhibition towards Studies Related to Antimicrobial Resistance
Project Supervisors: Dr. Paolo Bertoncello (Engineering) and Prof. Diane Kelly (Medicine)
This is a non-funded PhD opportunity available at Swansea University’s College of Engineering and based in the College of Engineering.
Electrochemiluminescence (ECL) is a kind of luminescence produced as a result of electrochemical reactions. ECL is a process in which electrochemically generated species combine to undergo highly-energetic electron transfer (redox or enzymatic) reactions that emit light from excited states. ECL has several attractive features, including the absence of a background optical signal, precise control of reaction kinetics offered by controlling the applied potential, and compatibility with solution-phase as well as thin-film formats. As such, ECL is a very versatile and useful electroanalytical technique to analyse and quantify enzymatic processes.
This is a collaborative project between Dr. Paolo Bertoncello (Engineering) and Prof. Diane Kelly (Medicine).
Aims of the project:
The main objective of this proposal is the fabrication of CYP51A1 and polymer/CYP51A1 composite thin films and their deposition on electrode substrates for ECL studies. These ECL studies will provide the basis for the development of a novel electrochemiluminescence biosensor for azoles detection, as well providing insights into the mechanisms of ECL quenching operated by azoles in the presence of NADH and H2O2 as coreactants. The ECL studies of CYP51A1 herein performed go beyond their electroanalytical applications, since they provide useful insights to understand mechanisms of antimicrobial resistance (AMR) with obvious beneficial repercussions in the medical and biotechnology sectors.
Candidates must have a first, upper second class honours or a Master’s degree (with Merit) in a relevant discipline. This project will require a background in chemistry/materials science, with strength in electrochemical characterisation methods. Knowledge of deposition of thin films using the Langmuir-Blodgett/Langmuir-Schaefer methods would also be desirable, but is not essential.
Please note that this is a self-funded PhD project.