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Simultaneous gene transcription and translation in bacteria

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  • Full or part time
    Dr Gabriel Demo
  • Application Deadline
    No more applications being accepted
  • Funded PhD Project (Students Worldwide)
    Funded PhD Project (Students Worldwide)

Project Description

OBJECTIVES: The research aims to unravel the fundamental mechanism of the transcription-translation coupling in bacteria. These studies will significantly advance the current understanding how the lead ribosome closely trails the RNA polymerase (RNAP), thus promoting pause-free transcription, mRNA quality and efficient gene expression in bacteria.

FOCUS: Doctoral research projects focus on the mechanistic details and functional outcomes of coupled transcription-translation in bacteria. Students benefit from the shared cutting-edge core facilities of CEITEC that include (i) X-ray crystallography – crystallization robot Mosquito, Rigaku crystal hotel and diffraction system, (ii) cryo-EM equipment - Versa 3D dual beam microscope, Titan Krios and F20 electron microscopes (iii) High-field NMR systems, and (iv) biomolecular interactions equipment - confocal microscopes, near-field optical microscope (SNOM), surface plasmon resonance, microcalorimetric equipment.

The applicant may focus on one of these areas (for details see: http://ls-phd.ceitec.cz/dissertation-topics-current-call/
Structural and functional identification of factors required to couple transcription and translation in bacteria
Structural studies of various states of direct and bridged transcription-translation coupling in vitro and in vivo

We are seeking a Ph.D. candidate who holds MSc. in molecular biology or biochemistry and run the biochemical assays, or who was trained in structural biology, mainly single-particle cryo-EM or cryo-electron tomography (cryo-ET). The candidate should be able to purify the components for structural studies of transcription-translation coupling and run the biochemical assays. The candidate should be a motivated person with collaborative mindset.

HOW TO APPLY:
• To apply please contact the supervisor and submit a CV by email to: [Email Address Removed] and [Email Address Removed] (Subject: PhD School).
• Information about the research group at: https://www.ceitec.eu/structural-biology-of-coupled-transcription-translation-gabriel-demo/rg352

Funding Notes

Admission for studies, student registration for the full-time study program, and proper fulfillment of student duties constitute the student’s right to a regular income of 22 000 CZK (850 EUR). Information is available at http://ls-phd.ceitec.cz/information-for-students/#fund
Living costs and other practicalities available at http://ls-phd.ceitec.cz/practical-information-for-life-in-brno/
Brno is one of the major cultural hubs, with a vibrant and lively atmosphere housing ~60.000 students. The city has a very good public transport and plenty of interesting places to visit within the reach of trains (within small distance of several major cities such as Prague, Vienna, Bratislava, Budapest) and close to international airports.



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