Simultaneous gene transcription and translation in bacteria

OBJECTIVES: The research aims to unravel the fundamental mechanism of the transcription-translation coupling in bacteria. These studies will significantly advance the current understanding how the lead ribosome closely trails the RNA polymerase (RNAP), thus promoting pause-free transcription, mRNA quality and efficient gene expression in bacteria.

FOCUS: Doctoral research projects focus on the mechanistic details and functional outcomes of coupled transcription-translation in bacteria. Students benefit from the shared cutting-edge core facilities of CEITEC that include (i) X-ray crystallography – crystallization robot Mosquito, Rigaku crystal hotel and diffraction system, (ii) cryo-EM equipment - Versa 3D dual beam microscope, Titan Krios and F20 electron microscopes (iii) High-field NMR systems, and (iv) biomolecular interactions equipment - confocal microscopes, near-field optical microscope (SNOM), surface plasmon resonance, microcalorimetric equipment.
The applicant may focus on one of these areas:
Structural and functional identification of factors required to couple transcription and translation in bacteria
Structural studies of various states of direct and bridged transcription-translation coupling in vitro and in vivo

We are seeking a Ph.D. candidate who holds MSc. in molecular biology or biochemistry and run the biochemical assays, or who was trained in structural biology, mainly single-particle cryo-EM or cryo-electron tomography (cryo-ET). The candidate should be able to purify the components for structural studies of transcription-translation coupling and run the biochemical assays. The candidate should be a motivated person with collaborative mindset.

HOW TO APPLY:
• To apply please contact the supervisor and submit a CV by email to: [Email Address Removed]
• Information about the research group at: https://www.ceitec.eu/structural-biology-of-coupled-transcription-translation-gabriel-demo/rg352