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Simultaneous imaging of protein-protein and protein-membrane interactions using molecular rotors

   Institute of Chemical Biology

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  Dr M Kuimova, Dr A Barnard, Prof B Byrne  Applications accepted all year round  Funded PhD Project (Students Worldwide)

About the Project

Protein-protein interactions (PPIs) are integral to all biological processes, including those involved in disease pathways. However, methods to study and quantify the effect of targeting these interactions in real time and at a single cell level are currently lacking. This project will develop an exciting opportunity to use molecular rotors, environmentally sensitive fluorophores, to quantitatively detect the function of a PPI inhibitor as a large change in fluorescence lifetime. We will develop novel peptide-rotor conjugates as tools to study the Bcl-2 family of PPIs, key oncology targets. Additionally, inhibition of these PPIs is inexorably linked to permeabilisation of the mitochondrial outer membrane. By employing rotors with complementary emission properties, we will develop methods to study both PPI inhibition and membrane integrity simultaneously, offering unprecedented insight into these interlinked biological events.

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