Lectins are carbohydrate-binding proteins that control various functions in living organisms including the cell-cell interaction, inflammatory and immune response, fertilization, adhesion and virulence of pathogens. Particularly galectins (defined by their binding specificity for galactosides) are in the center of many research studies as immune checkpoints in pathogenesis, or tumor progression and metastasis. The ability to design new galectin inhibitors that would suppress these negative processes envisages not only understanding the carbohydrate-lectin structure but also requires knowing the conformational behavior of the inhibitor in an unbound state. In the thesis, we propose to prepare carbohydrate conjugates bearing metal tags that allow us to measure paramagnetic NMR pseudo-contact shifts (PCS) and circularly polarized luminescence (CPL) sensitive to the conformation and to close environment. The interpretation of the experiment is not straightforward and theoretically based predictions will be developed, to understand the spectral parameters.
How to apply
To apply for a PhD study at IOCB Prague, you must hold a Master’s degree (MSc) or the equivalent of the MSc in similar field (four or five year undergraduate degree). The application can be submitted before obtaining the Master’s degree, however, the applicant should obtain the degree within five months after the application deadline.
For more information visit our website “Call for PhD applications 2020”: https://www.uochb.cz/en/phd-program
If you decided to apply, please do so online via our application form: https://www.uochb.cz/en/call-for-phd-applications
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Canales, A. et al., Angew. Chem. Int. Ed. 2017, 56, 14987. “Breaking the Limits in Analyzing Carbohydrate Recognition by NMR Spectroscopy: Resolving Branch-Selective Interaction of a Tetra-Antennary N-Glycan with Lectins.”