Postgrad LIVE! Study Fairs

Birmingham | Edinburgh | Liverpool | Sheffield | Southampton | Bristol

University of Birmingham Featured PhD Programmes
Imperial College London Featured PhD Programmes
Imperial College London Featured PhD Programmes
Coventry University Featured PhD Programmes
Birkbeck, University of London Featured PhD Programmes

Structural and Functional Characterisation of TRIM25-RNA Complex

  • Full or part time

    Dr A Cook
  • Application Deadline
    Friday, February 01, 2019
  • Competition Funded PhD Project (European/UK Students Only)
    Competition Funded PhD Project (European/UK Students Only)

Project Description

RNA viruses continue causing devastating epidemics and pandemics in the 21st century. For example, influenza A virus kills 250,000 to 500,000 people annually and generates huge socioeconomic burden. The first line of defence against RNA viruses is the innate immune system. TRIM25 is a key factor in this process. It enables innate immune response to RNA viruses by activating key host anti-viral pathways.

Using our expertise in RNA biology we discovered that TRIM25 binds to RNA. We hypothesise that during infection by RNA viruses, TRIM25 senses and restricts the virus by binding to specific viral RNAs. However, detailed structure of TRIM25/RNA complex is still missing.

The aim of this PhD is to take advantage of Michlewski’s (Division of Infection and Pathway Medicine) and Cook’s (Wellcome Centre for Cell Biology) expertise in functional and structural characterization of RNA-protein complexes, respectively. The PhD student will establish the three-dimensional structure of TRIM25/RNA complex and use this information to engineer cells with TRIM25 mutations that prevent it from binding to RNA. He/she will test the innate immune response of these cells to infection with influenza A. The outcome of this research will reveal how TRIM25 uses its RNA-binding activity for anti-viral functions and will provide a platform for the development of novel, RNA-based anti-viral therapeutics.

Funding Notes

Home/EU fees, student’s stipend £15.5k - £16.5k and £8k consumables per yer.

All candidates should have or expect to have a minimum of an appropriate upper 2nd class degree or equivalent.

CV, cover letter and details of two academic referees should be sent to

References

Gasparini, R. et al. Clinical and socioeconomic impact of seasonal and pandemic influenza in adults and the elderly. Hum Vaccin Immunother 8, 21-8 (2012).

Meyerson, N.R. et al. Nuclear TRIM25 Specifically Targets Influenza Virus Ribonucleoproteins to Block the Onset of RNA Chain Elongation. Cell Host Microbe 22, 627-638 e7 (2017).

Choudhury, N.R. et al. RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC Biol 15, 105 (2017).

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2018
All rights reserved.