About the Project
Recently we characterised the structure of a domain in MOZ (the double PHD finger) can bind and acetylate the Histone H3 tail and induce its alpha helical conformation and acetylate H3K9 and H3K14 in chromatin (Dreveny et al., NAR 2014).We have now defined additional functional domains with novel roles in chromatin recognition. The project will involve characterising these novel functions using structural analysis (X-ray crystallography) and mutagenesis. Using cancer cell lines depleted of MOZ by CRISPR CAS9 editing, RNA-Seq was used to establish transcriptomes and thus MOZ-regulated genes (manuscripts in preparation). This project will also involve rescue experiments with wild type and mutant MOZ proteins, to enable us to understand the role of novel MOZ domains in gene regulation and in leukemias.
The project will involve the use of molecular and cell biology techniques, including protein purification and structural analysis, supported by gene expression, chromatin binding studies and confocal microscopy.
The project will be carried out within the Gene Regulation & RNA Biology Group (circa 30 researchers) within the Division of Molecular & Cellular Sciences. The School of Pharmacy provides a vibrant cross-disciplinary research environment, with excellent support and training facilities for PG students.
Why not add a message here
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.