About the Project
However, most single-cell transcriptomic methods just allow sequencing of the 3’UTR ends of mRNAs. This prevents the analysis of many important cellular processes such as alternative splicing, RNA editing and alternative polyadenylation. Developing a method that can profile full-length mRNAs with single-cell resolution is an urgent need to establish the roles of those processes in cell differentiation, development and disease.
To establish this method, the Solana laboratory is teaming up with Oxford Nanopore Technologies (ONT). The Solana laboratory has expertise in single-cell transcriptomics. ONT is a world leader in long read RNA and DNA sequencing. The student will exploit and combine both of these technologies to develop a method that can profile mRNAs at full-length resolution in thousands of single cells. Once established, the student will use this method to study the role of alternative splicing, RNA editing and alternative polyadenylation in planarian stem cell differentiation. This will reveal new insights in stem cell biology and animal regeneration.
Attributes of suitable applicants:
The candidate should have a degree from a Higher Education Institution in the UK or acceptable equivalent qualification in biology or related subject.
This project is supported through the Oxford Interdisciplinary Bioscience Doctoral Training Partnership (DTP) studentship programme. The student recruited to this project will join a cohort of students enrolled in the DTP’s interdisciplinary training programme, and will be able to take full advantage of the training and networking opportunities available through the DTP. For further details please visit www.biodtp.ox.ac.uk. NOTE: This student will be enrolled at Oxford Brookes University (https://www.brookes.ac.uk/bms/research/degrees/research-opportunities/).
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