The aim of this project is to investigate cellular mechanisms of surfactant secretion in lung alveoli and their disruption in pulmonary disease. Surfactant is a hydrophobic substance that enables breathing and is secreted from alveolar type II cells into alveolar lumen via exocytosis. Surfactant deficiency can cause infant respiratory distress syndrome and pulmonary fibrosis; however, the molecular mechanisms of surfactant secretion in health and disease are not well known. This project will investigate surfactant secretion using methods such as cell culture of primary cells and lung epithelial cell lines, live cell microscopy, immunofluorescence and molecular biology.
This PhD is self-funded.
This project will profit from collaborations with the University Hospital of South Manchester and University of Manchester.