Phosphoinositide lipids (PIPs) play critical roles in development and the homeostasis of adult tissues and their dysfunction has been linked to major human diseases such as cancer, diabetes, myopathies and neuropathies. We are working on an enzyme called the Class II Pi3K which generates two distinct PIP species: PI3P and PI(3,4)P2. While roles for the PI3P signal have been described, cellular functions for PI(3,4)P2 have only recently begun to emerge from studies in cell lines.
This project will address the functions of PI(3,4)P2 in vivo within a whole organism the fruit fly, Drosophila. Since PIPs, the kinases that make them and the phosphatases that degrade them, are conserved between Drosophila and mammals, we can use Drosophila as a genetically tractable model system to understand the functions of PIP signals. This project will use mutants and RNAi to manipulate the levels of kinases and phosphatases to determine how the levels of PI(3,4)P2 are controlled. A range of genetic, biochemical and cell biological techniques will then be utilised including fluorescently-tagged lipid binding domains to visualise the PIP signals in vivo and understand the impact on protein trafficking of membrane proteins such as the Notch receptor.
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a related area / subject. Candidates with experience or an interest in Drosophila/signalling are encouraged to apply.
For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (https://www.bmh.manchester.ac.uk/study/research/apply/). Informal enquiries may be made directly to the primary supervisor.”
How To Apply
For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (https://www.bmh.manchester.ac.uk/study/research/apply/). Informal enquiries may be made directly to the primary supervisor. On the online application form select PhD Genetics
For international students, we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences.
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