About the Project
Capitalising on uniquely powerful genetic analyses of neurons of the fruit fly Drosophila in culture and in vivo, we have identified a number of key mechanisms that maintain axonal MT bundles in order. These involve MT polymerisation and guidance, their cortical elimination and their stabilisation, all essential to maintain parallel MT bundles. An important further class of regulators are MT severing proteins including spastin, katanin and fidgetin, which disassemble MTs and maintain them in a dynamic state. Of these, spastin is closely linked to spastic paraplegia, a motorneuron disease-like condition, and we find that also loss of katanin has detrimental effects on axons.
On this project you will study the important roles of MT-severing proteins and unravel how they contribute to axonal longevity and decay. For this, you will use genetic manipulations of Drosophila neurons in culture and in vivo, advanced microscopy and image analysis, electron microscopy, as well as basic molecular biology and biochemistry. Furthermore, Andreas Prokop is a science communication expert providing important further training possibilities relevant for your future career path.
Training/techniques to be provided:
Training opportunities will include classical genetics and its application in Drosophila, cell culture techniques, analyses of brain tissues in vivo, advanced microscopy including super-resolution and live imaging, advanced image analysis, electron microscopy, basic skills in molecular biology and biochemistry, as well as science communication
Candidates are expected to hold a minimum upper second class honours degree (or equivalent) in a biology subject. Candidates with a convincing interest in the subject matter are encouraged to apply. Some laboratory experience will help your application, but this can be discussed.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit http://www.internationalphd.manchester.ac.uk
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
• Prokop, A., Beaven, R., Qu, Y., Sánchez-Soriano, N. (2013). Using fly genetics to dissect the cytoskeletal machinery of neurons during axonal growth and maintenance. J. Cell Sci. 126, 2331-41 -- http://dx.doi.org/10.1242/jcs.126912
• Hahn, I., Voelzmann, A., Liew, Y.-T., Costa-Gomes, B., Prokop, A. (2019). The model of local axon homeostasis - explaining the role and regulation of microtubule bundles in axon maintenance and pathology bioRxiv, 10.1101/577320 -- https://www.biorxiv.org/content/10.1101/577320v3
• Illingworth, S., Prokop, A. (2017). Science communication in the field of fundamental biomedical research (editorial). Sem Cell Dev Biol 70, 1-9 -- https://tinyurl.com/yaj8gzrp
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