About the Project
G protein-coupled receptors, one of the most diverse and ubiquitous families of integral membrane proteins, play a pivotal role in many cellular signalling pathways. They are prime targets for the development of therapeutic agents designed to either block or activate these receptors. Although huge progress has been made in elucidating the structure of “Family A” GPCRs (e.g. β-adrengeric receptor structure led to the 2012 Nobel Prize in Chemistry), much less progress has been made with their cousins in “Family B”, despite their physiological importance and potential therapeutic potential for treating diabetes, migraine, pain, osteoporosis, etc.). We aim to elucidate the mechanism by which the Family B PTH2 receptor binds its ligand(s) and transduces a signal across the plasma membrane to initiate cell signalling. Techniques will include all general recombinant DNA work (subcloning, DNA preps, site-directed mutagenesis, etc), generation of recombinant baculovirus, over-expression and purification of receptor protein, transfection/culture of mammalian cells, stable cell line generation, radioligand binding assays, 384-well plate-based cell signalling assays, molecular modeling, crystallisation and crystallography.
4 year BBSRC studentship, under the White Rose Mechanistic Biology DTP. The successful applicant will receive fees and stipend (c.£14,057). The PhD will start in Oct 2016. Applicants should have, or be expecting to receive, a 2.1 Hons degree in a relevant subject. EU candidates must have been resident in the UK for 3 years in order to receive full support. There are 2 stages to the application process: www.fbs.leeds.ac.uk/postgraduate/phdopportunities.php
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