Lysosomes are vital organelles. Besides removing cellular waste, processing nutrients and destroying pathogens, they serve as hubs for signalling pathways. Their numbers increase greatly during cellular senescence, a cell state crucial in both cancer suppression and ageing. Better understanding of this phenomenon and the underlying mechanisms may have wide-ranging applications in degenerative diseases, cancer, infections and lysosomal disorders.
This interdisciplinary project will first exploit “big data” in functional genomics to understand the molecular networks regulating lysosomal gene expression in cell senescence and following infection.
Putative regulators so identified will then be studied in the laboratory to verify their functions. Experimental approaches will focus on using cultured cells engineered to overexpress or lack the relevant regulator genes.
SKILLS WE EXPECT A STUDENT TO DEVELOP/ACQUIRE WHILST PURSUING THIS PROJECT
** Recombinant DNA methods
** Mammalian cell culture
** Gene transfer and gene editing
** Reporter gene assays, lysosomal enzyme assays
** Inducing and assaying cell senescence
** RNA extraction, quantitative PCR
** Fluorescence microscopy
** Retrieval of large genomics datasets from public repositories
** Data analysis using R and Linux/Unix
** Analysis of genomics data (sequences, gene expression data, molecular interaction data) using R/Bioconductor
All necessary skills will be taught.
PRIOR EDUCATIONAL REQUIREMENTS
BSc/BA, 1st class or 2(i) in biomedical science, bioinformatics, molecular biology or related. Preferably also MRes/MSc/MA in similar field, distinction or merit.
HOW TO APPLY
Potential applicants are strongly encourage to contact the supervisors (contact information below).
To apply please follow the instructions on the MRC-LID site at http://mrc-lid.lshtm.ac.uk/apply/